Dear Customer,

Welcome to the latest edition of the raytest newsletter in which we are pleased to provide you with our latest product information.

 

 

BIOSPACE Photon IMAGER

New device for real-time bioluminescence imaging in moving rodents



Abstract. Bioluminescence imaging (BLI) allows detection of biological functions in genetically modified cells, bacteria, or animals expressing a luciferase (i.e. firefly, Renilla, or aequorin). Given the high sensitivity and minimal toxicity of BLI, in vivo studies on molecular events can be performed noninvasively in living rodents.

To date, detection of bioluminescence in living animals has required long exposure times that are incompatible with studies on dynamic signaling pathways or nonanaesthetised freely moving animals.

Here we develop an imaging system that allows:
  1. bioluminescence to be recorded at a rate of 25 images/s using a third generation intensified charge-coupled device (CCD) camera running in a photon counting mode, and
  2. coregistration of a video image from a second CCD camera under infrared lighting.
The sensitivity of this instrument permits studies with subsecond temporal resolution in nonanaesthetized and unrestrained mice expressing firefly luciferase and imaging of calcium signaling in transgenic mice expressing green fluorescent protein (GFP) aequorin. This imaging system enables studies on signal transduction, tumor growth, gene expression, or infectious processes in nonanaesthetized and freely moving animals.

Journal of Biomedical Optics 13.5., 054035 .September/October 2008

Click here to download the publication.
 
 
 

Please visit us at the following exhibition:

   11. - 15. Oct 2008
EANM´08
Munich, Germany
Booth F32
  
  
 
 

 
Austria, BeNeLux, Switzerland, France, Italy, Spain, Sweden, Denmark, Norway, Finland, United Kingdom, United States, Czech Republic, Poland, Turkey, Hong Kong, China, Japan, Korea, Saudi Arabia, United Arab Emirates and Quatar
 
 

If you do not wish to receive future editions of the raytest Newsletter, please click here to unsubscribe.

Impressum: Carsten  Dietzel
raytest Isotopenmessgeräte GmbH 
Benzstr. 4, 75334 Straubenhardt, Germany 
Phone: +49 (0) 7082 / 9255 - 0; fax: +49 (0) 7082 / 9255 - 4444
www.raytest.com ; info@raytest.com

Chief Executive Officers:
Carsten Dietzel, Dipl.Wi.Ing. (FH), MBA;  Sven-Oliver Dietzel, Dipl.Ing. (FH)

Court of Registration: Mannheim, Germany; HRB 501307
VAT-No. DE 144 600 194; Tax-No. 49047/02533