Sybr Green I and II  
  nucleic acid gel stains  
         
 
Sybr Green I and Sybr Green II nucleic acid gel stains are ultra sensitive gel stains that surpass conventional dyes, including ethidium bromide, by more than an order of magnitude in nucleic acid sensitivity.
   
 

Sensitive Detect as little as 20 pg dsDNA per band
High Contrast Bright green fluorescence with exceptionally low background
Easy to Use No destaining or washing steps
Versatile Can be used with many different electrophoresis platforms
Convenient Staining does not interfere with DNA modification enzymes
Economical Less expensive than silver staining


   
     
 

SYBR Green stain is extremely versatile and easy to use. It is compatible with many different electrophoresis platforms, including native and denaturing agarose and polyacrylamide gel electrophoresis, pulsed field gel electrophoresis and capillary electrophoresis. SYBR Green stain has an exceptionally high affinity for dsDNA, making it possible to stain dsDNA prior to electrophoresis. Furthermore, SYBR Green stain does not interfere with many enzymes used in molecular biology, including Taq DNA polymerase, reverse transcriptase, restriction endonucleases, and T4 DNA ligase. Finally, SYBR Green stain has been shown to be much less mutagenic than ethidium bromide in Ames tests.

 
     
 

SYBR Green stain lets you:

 

    
 
  • Detect rare PCR products - The superior sensitivity of SYBR Green stain makes it possible to detect rare amplicons, reduce cycle numbers for PCR and RT PCR and accurately quantitate low numbers of PCR products made during the linear portion of the reaction, facilitating high throughput and competitive PCR analysis.
   
         
 
  • Save money on DNA typing - SYBR Green stain is as sensitive as silver staining- but less expensive - for human identity determination, CAG repeat detection and mtDNA deletion analysis.
   
         
 
  • Use fewer cells to visualize apoptosis ladders - SYBR Green stain gives brighter signals and lower background than ethidium bromide, making it possible to visualize DNA ladders from fewer apoptotic cells.
   
         
 
  • Perform more sensitive telomerase activity assays - SYBR Green stain is more sensitive than silver staining for gel-based detection of telomerase activity, allowing simultaneous, non-isotopic measurement of both enzyme activity and processivity.
   
         
 
  • Eliminate radioactivity in DNA damage assays - SYBR Green stain is as sensitive as 3H-labeled thymidine for detecting DNA damage.
   
         
 
  • Perform real-time or kinetic PCR analysis - The presence of SYBR Green stain during PCR does not interfere with Taq polymerase or reverse transcriptase, and primers contribute little to the signal, allowing amplification to be monitored in real time.
   
     
 
  • Detect lower amounts of contaminating DNase - SYBR Green stain is at least ten times more sensitive than ethidium bromide for detecting DNase activity using gel-based or radial diffusion assays.
 
     
 
  • Detect dsDNA using capillary electrophoresis - The high affinity for dsDNA and the excellent signal to background ratio make SYBR Green stain the dye of choice for capillary electrophoresis studies.
  
     
     
 
Sybr Green I
Sybr Green II

SYBR Green I nucleic acid gel stain is ideal for detecting double-stranded DNA (dsDNA) in electrophoretic gels using laser scanners, CCD-based image documentation systems or standard Polaroid photography. Following electrophoresis, gels are simply stained in buffered dye solution. No destaining or wash steps are required.

SYBR Green II nucleic acid gel stain is ideal for single stranded DNA (ssDNA) and RNA in electrophoretic gels using laser scanners, CCD-based image documentation systems or standard Polaroid photography. Following electrophoresis, gels are simply stained in buffered dye solution. No destaining or wash steps are required.