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Images can be turned, flipped, enlarged and zoomed. The gray scale representation
of the image is given and can be changed in many ways to enhance the visibility
of the original data. Editable text and arrows in different fonts, size
and orientation can be added to the image as overlays. To comply with
GLP standard, the original image data are never changed. All images are
equipped with detailed information of their source, size etc.
click
here for an example of AIDA Basis |
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The 1D quantification is a
highly efficient and accurate tool. Typically, all types of 1D gel electrophore
experiments as well as Southern-, Northern- and Western blots are evaluated.
Densitometer windows can be drawn in any width or angle through the image.
Lanes, band, the bending of lanes and the backround can be detected automatically
or manually. The resulting profiles can be displayed in different layouts.
In a configurable result table single values or the sum of integrated
peaks can be set as 100%.
Calibration standards can be used by AIDA to convert the relative intensity
integrals to absolute measurements (e.g.: µg, nmol). The calibration
curve can be reviewed and adapted to fit various functions.
click
here for an example of AIDA 1D Quantification |
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Evaluates dot blots, microtiterplates,
histological sections or other complex 2D-structures. Regions such as
rectangles, squares, ellipses, circles, polygons, free drawing and auto
contour are defined as overlays and can be turned, resized and reformatted
in any way to improve results. Matrices can be defined, stored, reloaded
and reedited for quick and easy evaluation of dot blots, microtiterplates
and other well defined geometrical structures.
The backround can be assigned to each region or a local backround, surrounding
the region, can be substracted. Gray scale integration limits can be selected
for individual regions. The result table can be configured in many ways,
and the results of any regions can be linked to groups with an individual
backround. Any region value within a group can be set to 100%. Regions
can be easily renumbered or labelled with names. The generation of a name
table allows quick labelling.
click
here for an example of AIDA 2D Quantification |
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The size of DNA, the mass of proteins,
isoelectric points and RF values can be calculated from the migration
distance by the use of individual standards. The standards can easily
be stored in a library. Automated assignment of standard bands can be
started by simple clicking. When multiple standard lanes are present,
the running distortion (e.g: the smile effect of a gel) is automatically
calculated and displayed resulting in very precise and resprodicible results.
click
here for an example of AIDA Fragment Length Determination
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