Imaging Flow Cytometry Overview

       
 

Specifications

 

FlowSight

ImageStreamX

  FlowSight
Overview Compact, high-sensitivity, multi-color flow cytometer. Produces multiple images per cell for qualitative and limited quantitative image analysis. High-resolution microscope for suspended cells. Produces detailed brightfield, darkfield, and fluorescence imagery for a wide range of quantitative, statistically robust image-based assays.

Magnification 20X Fixed 60X / 40X / 20X
Pixel Area 2.0 square microns 0.1 / 0.25 / 1.0 square microns
Number of Channels 12 6 or 12 High Resolution
Max Field of View 64 microns 128 microns
Quantitative Image Analysis Optional Standard
Brightfield Illumination 2 Channels Standard 10 Channels Standard
488 Excitation Laser 50 mW 100 mW
Laser Power Doubler Optional Standard
375 Excitation Laser - Coming Fall '11
405 Excitation Laser 90 mW 120 mW
561 Excitation Laser 50 mW 200 mW
592 Excitation Laser - 300 mW
642 Excitation Laser 100 mW 150 mW
730 Excitation Laser - Coming Fall '11
785 Side Scatter Laser 10 mW 10 mW
Sample format microcentrifuge tube microcentrifuge tube
AutoSampler Optional Optional
Extended Depth of Field - Optional
Sensitivity 10 MESF 5 MESF
 

Applications

 

FlowSight

FlowSight

ImageStreamX

Flow Cytometry
Visual Image Confirmation
Traditional Flow Cytometry
Quantitative Image Analysis
Nuclear Translocation • *
Shape Change • *
Internalization / Phagocytosis • *
Apoptosis • *
Mitotic Index  
Co-localization  
Spot Count  
Autophagy  
Immune Synapse  
Conjugates vs Cell Fusions  
Morphology-based Cell Class  
Asymmetric Cell Division  
* with Quantitative Imaging upgrade