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In a multilabelling experiment, two or more probes are labelled with different
fluorophores, which have sufficiently different emission wavelengths to
distinguish them. This makes parallel processing of different samples
possible, which is one of the major advantages of fluorescence labelling
techniques. Many applications like array technology, proteomics, 1D-gelelctrophoresis,
fluorescence in situ hybridisation etc. benefit from this strategy. In
order to make use of the broad spectrum of different possible fluorphores,
detection systems should have flexibility in the excitation wavelength
as well as in the emission filters.
Highly
efficient multilabelling can be imaged with the CCD camera systems for In-Vivo & In-Vitro Imaging and the variable epiillumination unit, which
provides a maximum flexibility in fluorescence excitation.
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