Isotope Detection
 

The method of choice for isotope detection in digital autoradiography is the Image PlateTM (IP) technology. Imaging Plates is a reusable two-dimensional sensor for the detection and storage of ionizing radiation energy found in photo-stimulable phosphors, which consist of a complex matrix of BaFX:Eu2+ (X: halogen) crystals.
There are IPs for virtually any type of emitter, all with superior accuracy.
Due to the unmached sensitivity, the high dynamic range of linear response to the signals, the IP-technology delivers most accurate data in quantitative experiments. Frequently, IP technology is used for Southern and Northern Blot detection, array technology, histology and other techniques with needs for highest sensitivity.


 
 
Advantages of IP-technology:

   
 
  • reuseability of imaging plate, no film development necessary
  • dynamic range 5 orders of magnitude
  • linearity 5 orders of magnituden, digital data
  • direct acess to software evaluation
  • sensitivity relative to film10-1000 times more sensitive, depending on energy of emmitters
  • examples for absolute sensitivity:


    3H 100 dpm/mm2/h
    14C, 35S 0,9 dpm/mm2/h
      (5ß-particles/mm2/h)
    32P 0.11dpm/mm2/h
      (3ß-particles/mm2/h)
    125J 0,54 dpm/mm2/h