QWBR (Quantitative Whole Body Radioluminography) replaces the conventional autoradiography with organ resection and liquid scintillation counting for obtaining the data for ADME studies.
It has become an important technique for investigation of metabolism, especially for pharmacokinetic studies of new drugs to obtain quantitative data about the distribution of this substance and its by-products.
The main subject in quantitative whole-body radioluminography is the determination of how the compounds and its byproducts are distributed to the various organs and tissues.
Therefore, it is important that all interesting details of the animal section, even small ones, can be recognized at all. Also, to provide good quantitative
results the spill-over of the signal from the neighbourhood to a specified region should be as low as possible.
Imaging Plates are sensitive to exposition of radioactive radiation and are capable to store the obtained information. This can be read out by an imaging plate scanner and digitally stored on a computer's hard disk. The imaging plate is more linear, more sensitive and has a wider dynamic range than any previous detector for analyzing the radioactive content of whole-body sections.
Figure on the correlation of the tissue distribution data by quantitative whole-body autoradiography and those by classical method using liquid scintillation counting.
The regulatory authorities will accept radioluminography for quantitative tissue distribution studies as an alternative to classical method using organ resection and liquid scintillation counting, if the method is properly validated
Quotations, summaries, abstracts reffering to Radioluminography